Staphylococcus Aureus lan Methicillin-Resistant Staphylococcus Aureus
jeneng produk
HWTS-OT062 Staphylococcus Aureus lan Methicillin-Resistant Staphylococcus Aureus Kit Deteksi Asam Nukleat (Fluoresensi PCR)
Sertifikat
CE
Epidemiologi
Staphylococcus aureus minangka salah sawijining bakteri patogen penting infeksi nosokomial.Staphylococcus aureus (SA) kalebu staphylococcus lan minangka wakil saka bakteri Gram-positif, sing bisa ngasilake macem-macem racun lan enzim invasif.Bakteri kasebut nduweni karakteristik distribusi sing wiyar, patogenisitas sing kuat lan tingkat resistensi sing dhuwur.Gen nuklease termostabil (nuc) minangka gen staphylococcus aureus sing dikonservasi banget.
Saluran
| FAM | gen mecA tahan methicillin |
| ROX | Kontrol internal |
| CY5 | gen staphylococcus aureus nuc |
Parameter teknis
| Lumbung | ≤-18 ℃ & dilindhungi saka cahya |
| urip beting | 12 sasi |
| Tipe Spesimen | sputum, sampel infeksi kulit lan jaringan alus, lan sampel swab irung |
| Ct | ≤36 |
| CV | ≤5,0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL bakteri tahan methicillin.Nalika kit ndeteksi referensi LoD nasional, 1000/mL staphylococcus aureus bisa dideteksi |
| Kekhususan | Tes reaktivitas silang nuduhake yen kit iki ora duwe reaktivitas silang karo patogen pernapasan liyane kayata staphylococcus aureus sing sensitif methicillin, staphylococcus coagulase-negatif, staphylococcus epidermidis sing tahan methicillin, pseudomonas aeruginosa, escherichia coli, klebsiumaniella, pneumoniae mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Instrumen sing ditrapake | Applied Biosystems 7500 Sistem PCR Real-Time QuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P LightCycler®480 Sistem PCR Real-Time LineGene 9600 Plus Sistem Deteksi PCR Real-Time MA-6000 Real-Time Kuantitatif Thermal Cycler Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Pilihan 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. bisa digunakake karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Tambah 200µL saline normal menyang endapan sing diproses, lan langkah-langkah sabanjure kudu diekstrak miturut instruksi, lan volume elusi sing disaranake yaiku 80µL.
Pilihan 2.
Reagen Pelepasan Sampel Macro & Micro-Test (HWTS-3005-8) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tambah 1mL saline normal menyang endapan sawise dicuci nganggo saline normal, banjur aduk kanthi becik.Centrifuge ing 13.000r / min kanggo 5 menit, copot supernatant (cadangan 10-20μL supernatant), lan tindakake pandhuan kanggo ekstraksi sakteruse.
Reagen ekstraksi sing disaranake: Ekstraksi Asam Nukleat utawa Reagen Pemurnian (YDP302) dening Tiangen Biotech (Beijing) Co.,Ltd.Ekstraksi kudu ditindakake kanthi ketat miturut langkah 2 saka instruksi manual.Disaranake nggunakake banyu bebas RNase lan DNase kanggo elusi kanthi volume 100µL.
