15 Jinis-jinis Human Papillomavirus E6/E7 Gene mRNA
jeneng produk
HWTS-CC005A-15 Jinis Human Papillomavirus E6/E7 Gene MRNA Deteksi Kit (Fluoresensi PCR)
Epidemiologi
Kanker serviks minangka salah sawijining jinis kanker wanita sing paling umum ing saindenging jagad, lan kedadeyane ana hubungane karo virus papilloma manungsa (HPV), nanging mung sawetara infeksi HPV sing bisa berkembang dadi kanker.HPV beresiko dhuwur nginfèksi sel epitel serviks lan ngasilaké rong onkoprotein, E6 lan E7.Protein iki bisa mengaruhi macem-macem protein seluler (kayata protein suppressor tumor pRB lan p53), ndawakake siklus sel, mengaruhi sintesis DNA lan stabilitas genom, lan ngganggu respon imun antivirus lan antitumor.
Saluran
| Saluran | Komponen | Genotip diuji |
| FAM | Buffer Reaksi HPV 1 | HPV 16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Gen β-aktin manungsa | |
| FAM | Buffer Reaksi HPV 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Gene manungsa INS |
Parameter teknis
| Lumbung | Cairan: ≤-18 ℃ |
| urip beting | 9 wulan |
| Tipe Spesimen | sel exfoliated serviks |
| Ct | ≤38 |
| CV | ≤5,0% |
| LoD | 500 eksemplar/mL |
| Instrumen sing ditrapake | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Wektu Nyata Sistem PCR Real-Time SLAN-96P Sistem PCR LightCycler®480 Real-Time LineGene 9600 Plus Sistem Deteksi PCR Real-Time MA-6000 Real-Time Kuantitatif Thermal Cycler Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Reagen ekstraksi sing disaranake: Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3020-50-HPV15) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekstraksi kudu ditindakake kanthi ketat miturut pandhuan kanggo nggunakake .Volume elusi sing disaranake yaiku 50μL.Yen sampel ora rampung dicerna, bali menyang langkah 4 kanggo redigesting.Banjur tes miturut pandhuan kanggo nggunakake.
Reagen ekstraksi sing disaranake: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431).Ekstraksi kudu ditindakake miturut pandhuan kanggo nggunakake kanthi ketat (Ing langkah 5, kaping pindho konsentrasi solusi kerja DNaseI, yaiku, njupuk 20μL larutan saham RNase-Free DNaseI (1500U) menyang tabung centrifuge RNase-Free anyar, nambah 60μL buffer RDD, lan nyampur alon-alon).Volume elusi sing disaranake yaiku 60μL.Yen sampel ora rampung dicerna, bali menyang langkah 5 kanggo redigesting.Banjur tes miturut pandhuan kanggo nggunakake.
